abstract：:The energy dissipation/circulation function (EDCF) is the product of the specific energy dissipation rate in the impeller swept volume (P/kD(3)) and the frequency of particle circulation (1/t(c)) through that volume. A direct relationship between mycelial fragmentation and EDCF has been reported. However, and although...

journal_title：Journal of biotechnology

authors： Rocha-Valadez JA,Galindo E,Serrano-Carreón L

更新日期：2007-07-15 00:00:00

abstract：:We report on the isolation and biochemical characterization of a novel, cold-active and metagenome-derived lipase with a high stereo-selectivity for pharmaceutically important substrates. The respective gene was isolated from a cosmid library derived from oil contaminated soil and designated lipCE. The deduced aa sequ...

journal_title：Journal of biotechnology

authors： Elend C,Schmeisser C,Hoebenreich H,Steele HL,Streit WR

更新日期：2007-07-15 00:00:00

abstract：:Recombinant Volvariella volvacea endoglucanase 1 (EG1) and its catalytic module (EG1-CM) were obtained by expression in Pichia pastoris, purified by two-step chromatography, and the catalytic activities and binding capacities were compared. EG1 and EG1-CM exhibited very similar specific activities towards the soluble ...

journal_title：Journal of biotechnology

authors： Wu S,Ding S,Zhou R,Li Z

更新日期：2007-07-15 00:00:00

abstract：:The pyranose 2-oxidases from Trametes ochracea and Trametes pubescens share markedly similar amino acid sequences with identity of 93.4%. When expressed from the recombinant plasmids based on the same vector in the Escherichia coli host strain BL21(DE3) at higher growth temperatures, they differ strikingly in the form...

journal_title：Journal of biotechnology

authors： Maresová H,Palyzová A,Kyslík P

更新日期：2007-06-30 00:00:00

abstract：:Gene silencing experiments in difficult-to-transfect cells are often hampered by the presence of a background of untransfected cells. We present proof-of-concept data from two different strategies for enrichment of siRNA-transfected cells. In the first approach, a heterologous surface antigen is expressed from a plasm...

journal_title：Journal of biotechnology

authors： Narz F,Hübner S,Magyar S,Bielke W,Weber M,Dennig J

更新日期：2007-06-30 00:00:00

abstract：:Macroscopic modelling of bioprocesses requires the determination of a biological reaction scheme and a kinetic model. The a priori selection of an appropriate kinetic model structure is usually made difficult by the lack of detailed bioprocess knowledge and the profusion of apparently similar biological kinetic laws. ...

journal_title：Journal of biotechnology

authors： Grosfils A,Vande Wouwer A,Bogaerts P

更新日期：2007-06-30 00:00:00

abstract：:The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetr...

journal_title：Journal of biotechnology

authors： Wawrzynczyk J,Szewczyk E,Norrlöw O,Dey ES

更新日期：2007-06-30 00:00:00

abstract：:The Bacillus subtilis endoxylanase XynA (BSXY) is frequently used to improve the functionality of arabinoxylan-containing material in cereal based industries. The presence of endogenous Triticum aestivum xylanase inhibitors (TAXI-I and TAXI-II) in wheat is a real concern as they have a direct negative impact on the ef...

journal_title：Journal of biotechnology

authors： Bourgois TM,Nguyen DV,Sansen S,Rombouts S,Beliën T,Fierens K,Raedschelders G,Rabijns A,Courtin CM,Delcour JA,Van Campenhout S,Volckaert G

更新日期：2007-05-31 00:00:00

abstract：:Primary human lymphocytes and macrophages are an important target cells for human immunodeficiency virus (HIV). For targeted gene transfer into CD4(+) lymphocytes and macrophages, we constructed HIV vectors with envelope glycoprotein (gp120) from the T-cell tropic BH10 strain and the macrophage tropic SF162, and devel...

journal_title：Journal of biotechnology

authors： Miyake K,Miyake N,Shimada T

更新日期：2007-05-01 00:00:00

abstract：:One of the main limitations in bacterial polyhydroxyalkanoate (PHA) production with mixed cultures is the fact that primarily polyhydroxybutyrate (PHB) homopolymers are generated from acetate as the main carbon source, which is brittle and quite fragile. The incorporation of different 3-hydroxyalkanoate (HA) component...

journal_title：Journal of biotechnology

authors： Dai Y,Yuan Z,Jack K,Keller J

更新日期：2007-05-01 00:00:00

abstract：:A novel methodology based on analysis of mtDNA-cytb diagnostic sites was performed to discriminate four closely related species of Thunnus (Thunnus alalunga, Thunnus albacares, Thunnus obesus and Thunnus thynnus) and one species of Euthynnus (Katsuwonus pelamis) genus in raw and canned tuna. The primers used in the pr...

journal_title：Journal of biotechnology

authors： Bottero MT,Dalmasso A,Cappelletti M,Secchi C,Civera T

更新日期：2007-05-01 00:00:00

abstract：:Heparan sulfate (HS) is a cell surface carbohydrate polymer modified with sulfate moieties whose highly ordered composition is central to directing specific cell signaling events. The ability of the cell to generate these information rich glycans with such specificity has opened up a new field of "heparanomics" which ...

journal_title：Journal of biotechnology

authors： Lamanna WC,Kalus I,Padva M,Baldwin RJ,Merry CL,Dierks T

更新日期：2007-04-30 00:00:00

abstract：:Continuous photoproduction of H(2) by the green alga, Chlamydomonas reinhardtii, is observed after incubating the cultures for about a day in the absence of sulfate and in the presence of acetate. Sulfur deprivation causes the partial and reversible inactivation of photosynthetic O(2) evolution in algae, resulting in ...

journal_title：Journal of biotechnology

authors： Kosourov S,Patrusheva E,Ghirardi ML,Seibert M,Tsygankov A

更新日期：2007-03-10 00:00:00

abstract：:Cutinase from Fusarium solani pisi was genetically modified near the active site, by site-directed mutagenesis, to enhance its activity towards polyethylene terephthalate (PET) and polyamide 6,6 (PA 6,6) fibers. The mutations L81A, N84A, L182A, V184A and L189A were done to enlarge the active site in order to better fi...

journal_title：Journal of biotechnology

authors： Araújo R,Silva C,O'Neill A,Micaelo N,Guebitz G,Soares CM,Casal M,Cavaco-Paulo A

更新日期：2007-03-10 00:00:00

abstract：:Production of heterologous proteins in bacteria is one of the main applications of biotechnology. Although several high-efficiency expression systems have been developed, different steps in protein production may become rate-limiting depending on the production system and the protein being produced. One bottle neck ca...

journal_title：Journal of biotechnology

authors： Hienonen E,Romantschuk M,Fenel F,Taira S

更新日期：2007-02-01 00:00:00

abstract：:An expression vector has been generated using a gene highly expressed under conditions found in a typical fed-batch bioreactor process. The ferritin heavy chain (HC) gene exhibits higher levels of expression in the late stages of a fed-batch bioreactor than in the early stages. This property was considered advantageou...

journal_title：Journal of biotechnology

authors： Prentice HL,Tonkin CJ,Caamano L,Sisk WP

更新日期：2007-01-30 00:00:00

abstract：:Real-Time PCR has been applied to quantify extraradical soil mycelium of the edible ectomycorrhizal fungus Lactarius deliciosus in an interspecific competition experiment under greenhouse conditions. Couples of Pinus pinea seedlings inoculated with either L. deliciosus, Rhizopogon roseolus, or non-inoculated (control)...

journal_title：Journal of biotechnology

authors： Parladé J,Hortal S,Pera J,Galipienso L

更新日期：2007-01-30 00:00:00

abstract：:Epigallocatechin-3-O-gallate (EGCg) and related polyphenolic compounds found in tea are known to have antioxidative activities. However, they also have pro-oxidative activities such as generation of hydrogen peroxide. In this report, we investigated the effect on cells and showed the potential usage of EGCg in cell pr...

journal_title：Journal of biotechnology

authors： Matsumura K,Kim JY,Tsutsumi S,Hyon SH

更新日期：2007-01-20 00:00:00

abstract：:The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the development and exploitation of modern biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of ...

journal_title：Journal of biotechnology

authors： Yin J,Li G,Ren X,Herrler G

更新日期：2007-01-10 00:00:00

abstract：:A simple and accurate analysis system for the estimation of recombination efficiency in vivo using fluorescence-activated cell sorting (FACS) was designed and was subsequently used to compare the efficiency of recombination related to different spacer mutants. F(3) and F(5) mutant sequences were used for Flpe-mediated...

journal_title：Journal of biotechnology

authors： Kim MS,Kim WH,Lee GM

更新日期：2007-01-10 00:00:00

abstract：:Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumno rosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) ...

journal_title：Journal of biotechnology

authors： Maghuly F,da Câmara Machado A,Leopold S,Khan MA,Katinger H,Laimer M

更新日期：2007-01-01 00:00:00

abstract：:Randomly amplified polymorphic DNA (RAPD) was used to analyzed 78 samples comprises of certified reference materials (soya and maize powder), raw seeds (soybean and maize), processed food and animal feed. Combination assay of two arbitrary primers in the RAPD analysis enable to distinguish genetically modified organis...

journal_title：Journal of biotechnology

authors： Yoke-Kqueen C,Radu S

更新日期：2006-12-15 00:00:00

abstract：:The two main sugars in the agricultural by-product corn stover are glucose and xylose. Co-fermentation of glucose and xylose at high content of water-insoluble solids (WIS) without detoxification is a prerequisite to obtain high ethanol concentration and to reduce production costs. A recombinant strain of Saccharomyce...

journal_title：Journal of biotechnology

authors： Ohgren K,Bengtsson O,Gorwa-Grauslund MF,Galbe M,Hahn-Hägerdal B,Zacchi G

更新日期：2006-12-01 00:00:00

abstract：:A novel method that relies on the decoupling of the energy production and biosynthesis processes was used to characterise the maintenance, cell lysis and growth processes of Nitrosomonas sp. A Nitrosomonas culture was enriched in a sequencing batch reactor (SBR) with ammonium as the sole energy source. Fluorescent in ...

journal_title：Journal of biotechnology

authors： Vadivelu VM,Keller J,Yuan Z

更新日期：2006-11-10 00:00:00

abstract：:Intracellular and extracellular proteome analysis was carried out by combined two-dimensional gel electrophoresis and mass spectrometric analysis (2DE/MS) for high cell density fed-batch culture of recombinant Bacillus megaterium strains. In the early feeding phase with a constant growth rate of 0.12h(-1) under glucos...

journal_title：Journal of biotechnology

authors： Wang W,Sun J,Hollmann R,Zeng AP,Deckwer WD

更新日期：2006-11-10 00:00:00

abstract：:Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP6) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe ...

journal_title：Journal of biotechnology

authors： Cho J,Choi K,Darden T,Reynolds PR,Petitte JN,Shears SB

更新日期：2006-11-01 00:00:00

abstract：:This study aimed at increasing the pyruvate productivity from a multi-vitamin auxotrophic yeast Torulopsis glabrata, by increasing the availability of NAD+. We examined two strategies for increasing availability of NAD+. To supplement nicotinic acid (NA), the precursor of NAD+; and to increase the activity of alcohol ...

journal_title：Journal of biotechnology

authors： Liu L,Li Y,Shi Z,Du G,Chen J

更新日期：2006-11-01 00:00:00

abstract：:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich d...

journal_title：Journal of biotechnology

authors： Noda T,Kawamura R,Funabashi H,Mie M,Kobatake E

更新日期：2006-11-01 00:00:00

abstract：:POMT-7, an O-methyltransferase from poplar (Populus deltoids) was used to modify a variety of flavonoid compounds. POMT-7 was able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. However, POMT-7 showed a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteoli...

journal_title：Journal of biotechnology

authors： Kim BG,Kim H,Hur HG,Lim Y,Ahn JH

更新日期：2006-11-01 00:00:00

abstract：:Three of the most abundant proteins (OmpW, MopB and SodM) of the extracellular proteome of Xanthomonas campestris pv. campestris were analysed in a luminol-based oxidative burst assay to identify novel pathogen-associated molecular patterns (PAMP). Tobacco cell suspension cultures were used as a model system to monito...

journal_title：Journal of biotechnology

authors： Watt SA,Tellström V,Patschkowski T,Niehaus K

更新日期：2006-10-20 00:00:00

abstract：:Two-thirds of Earth's surface is covered by oceans, yet the study of this massive integrated living system is still in its infancy. Various environmental variables, such as high salinity, low and changeable nutrient availability and depth-correlated gradients of light, temperature, nutrients and pressure shape the div...

journal_title：Journal of biotechnology

authors： Simonato F,Campanaro S,Lauro FM,Vezzi A,D'Angelo M,Vitulo N,Valle G,Bartlett DH

更新日期：2006-10-20 00:00:00

abstract：:The genus Listeria comprises a group of non-sporulating, Gram-positive, soil bacteria belonging to the low G+C group of microorganisms. The genus consists of only six species, L. monocytogenes, L. ivanovii, L. seeligeri, L. innocua, L. welshimeri, and L. grayi.L. monocytogenes and L. ivanovii are the only known pathog...

journal_title：Journal of biotechnology

authors： Hain T,Steinweg C,Chakraborty T

更新日期：2006-10-20 00:00:00

abstract：:Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viab...

journal_title：Journal of biotechnology

authors： Talebnia F,Taherzadeh MJ

更新日期：2006-09-18 00:00:00

abstract：:Our unpublished experimental results of fractional factorial experiments showed that the significant external factors affecting high pressure processing (HPP) inactivation were pressure, temperature and pressure holding time. Based on these results, response surface methodology (RSM) was employed in the present work a...

journal_title：Journal of biotechnology

authors： Gao YL,Ju XR,Jiang HH

更新日期：2006-09-18 00:00:00

abstract：:Enzymatic hydrolysis constitutes an attractive strategy for biorefining of abundant, low-cost agricultural by-products such as wheat bran and straw. However, to adopt such an approach, efficient enzymes are required, in particular xylanases. To promote heat-induced disorganization of the complex cell wall network in w...

journal_title：Journal of biotechnology

authors： Paës G,O'Donohue MJ

更新日期：2006-09-18 00:00:00

abstract：:The alpha-amylase (1, 4-alpha-d-glucanohydrolase; EC 3.2.1.1) and alpha-glucosidase (alpha-d-glucoside glucohydrolase; EC 3.2.1.20) secreted by Geobacillus thermodenitrificans HRO10 were purified to homogeneity (13.6-fold; 11.5% yield and 25.4-fold; 32.0% yield, respectively) through a series of steps. The molecular w...

journal_title：Journal of biotechnology

authors： Ezeji TC,Bahl H

更新日期：2006-08-20 00:00:00

abstract：:Izumoring is a new concept for the bioproduction of all hexose sugars - 16 aldohexoses, eight ketohexoses and 10 hexitols -- using enzymatic and microbiological reactions. The biocatalysts necessary for the strategy were (i) d-tagatose 3-epimerase [which epimerizes all ketohexoses at C-3 of the sugar], (ii) polyol deh...

journal_title：Journal of biotechnology

authors： Izumori K

更新日期：2006-08-05 00:00:00

abstract：:Following the discovery of RNA interference (RNAi) and related phenomena, novel regulatory processes, attributable to small non-protein-coding RNAs, continue to emerge. Capitalizing on the ability of artificial short interfering RNAs (siRNAs) to trigger degradation of specific target transcripts, and thereby silence d...

journal_title：Journal of biotechnology

authors： Malphettes L,Fussenegger M

更新日期：2006-08-05 00:00:00

abstract：:Recombinant FVIIa has been developed for treatment of bleedings in hemophilia patients with inhibitors, and has been found to induce hemostasis even during major surgery such as major orthopedic surgery. Recombinant FVIIa is being produced in BHK cell cultures and has been shown to be very similar to plasma-derived FV...

journal_title：Journal of biotechnology

authors： Hedner U

更新日期：2006-08-05 00:00:00

abstract：:Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide poly...

journal_title：Journal of biotechnology

authors： Gharizadeh B,Akhras M,Nourizad N,Ghaderi M,Yasuda K,Nyrén P,Pourmand N

更新日期：2006-07-25 00:00:00